gel filtration

Christian Leymann leymann at
Mon Sep 14 20:37:27 EST 1998

On Mon, 07 Sep 1998 15:28:58 GMT, Dima Klenchin wrote:

:>In article <35F260B4.5828 at>, Ina Hinners 
<hinners at> wrote:
:>>Hi netters,
:>>I have a problem wich is quiet embarassing: I managed to run a
:>Unlike Sepharoses, Sephacryl is somewhat more resistant to drying.
:>By rehydrating, you can get usable column (say, if it is crude preparative
:>separation). However, there is no doubt that there is some irreversible
:>damage to the matrix and that such column will not be appropriate for
:>demanding applications . Personally, I would throw it away and pack 
:>a new column regardless.
:>        - Dima

In our german laboratory we could not afford to throw away the two bottles Sephacryl in a 
huge gel filtration column. We ran it for i while (5 volumes or so) with 70% ethanol and then 
applied a gradient from 70% ethanol to pure water. Then the column was opened, the 
material equilibrated in buffer and the column prepared as if the sephacryl was fresh...
and it worked quite fine.


More information about the Methods mailing list