Proteases in liver cytosols
leymann at gmx.net
Mon Sep 14 20:20:17 EST 1998
On 10 Sep 1998 11:08:11 -0700, Mike Green wrote:
:> A simple question regarding the preparation of liver cytosols? I use
:>10 mM phosphate, 150 mM NaCl to prepare uterine cytosols with no problem
:>of degradation. Will this same buffer work for liver tissue or are
:>protease inhibitors needed? Thanks for your input.
As far as I remember in liver is elevated phosphatase activity. This is no problem as long
as you don't need a phosphorylated protein. In this case glycerophosphate helps.
Preparing cytosol I always added 50 microg/ml phenylmethylsulfonylfluorid to inhibit
Hope this helps,
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