Is 260:280 ratio good for oligos?

Rich Dudley rdudley+ at pitt.edu
Mon Sep 14 09:48:01 EST 1998


I purchased some oligos, and haven't had any success at PCR.  Controls
pointed to the oligos as the problem.  I extracted the oligos twice with
n-butanol, and EtOH precipitated, and resuspended.  I quantitated at
260, but out of habit, I also took the 260:280 ratio.  Were these
midipreps, I would throw out the DNA and start over--ratios ranged from
1.1 to 1.4.  Does the 260:280 definition of "clean" hold for oligos, or
are these oligos probably ok to use?

Thanks!
rich

--
--- --- --- -- -- -- --- --- ---
Richard J. Dudley (rdudley+ at pitt.edu)
Research Specialist V
Dept. of Cell Biology and Physiology
University of Pittsburgh
http://www.cbp.pitt.edu
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