alternatives to immunization with gel band

tronni at my-dejanews.com tronni at my-dejanews.com
Tue Sep 15 23:42:09 EST 1998


In article <35FECB0D.553F61FC at acsu.buffalo.edu>,
  jnv at ACSU.BUFFALO.EDU (Josh VanHouten) wrote:
> Can anyone suggest good alternatives to immunizing animals with a
> protein band cut out of a polyacrylamide gel?
>

If you have an access to preparative SDS-PAGE apparatus by Bio-Rad
(called "Prep-Cell") and a fraction collector, that is a good bet.
You can load up to 6 milligrams
of total protein and run the tube gel overnight, collecting fractions
in Laemmli running buffer. Works nicely after small optimization.

Best regards

Dr. Tapani Ronni
UCLA/Howard Hughes Medical Institute

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