Any problems with Pfu Turbo

Nils Wichmann nwichmann at dermatology.uni-kiel.de
Tue Sep 15 09:52:47 EST 1998


Hi,
I always had good experinces with Pfu Turbo. I typically amplify products of
0,5 to 1 kb and the yields are comparable to Taq. I can only recommend it.

Nils

January Weiner wrote:

> We switched to Pfu - Turbo from Stratagene and seem to have many problems
> with it. Under the specified conditions (te=1min/kbp, template 0,5 ug
> genomic bacterial DNA) sometimes there is no product at all, even though it
> ought to be quite small (~3.5 kb). The only non-standard thing is that one
> of the oligonucleotides has a coupple of nucleotides more bearing a
> restriction site.
>         What are your experiences with Pfu Turbo?
>         Thank you,
>                         January
>
> --
> ----)-\//-///-----------------------------------January-Weiner-3-------
> A machine is only a machine because it cannot think - G.K.Chesterton






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