northerns and pre mRNA

Ruth McMahon Ruth.McMahon at ucd.ie
Wed Sep 16 07:08:56 EST 1998


When doing Northerns, is there an assumption that only mRNA will be
detected (when probing with a cDNA probe to total RNA)? If you have pre
mRNA with introns small enough, or even partially spliced pre mRNA, such
that it can still be picked up with your probe, is there a reason why
this does not happen, or does it happen? (Is pre mRNA/partially spliced
mRNA too transient in all situations?)
Thanks for any clarification,

Ruth.

--




Ruth McMahon, Ph.D.,                                Tel: 353-1-706-2815
Biotechnology Centre,                               Fax: 353-1-269-2016
University College Dublin,
Belfield,
Dublin 4,
Ireland.





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