IS-PCR + ISH question

Pharmacology channels at
Thu Sep 17 08:28:02 EST 1998

I am planning to do ISH or IS-PCR followed by ISH on guinea pig ileum
tissue sections to look for an ion channel.
I have tried this before with a DIG-labelled riboprobe without
significant results.
Any good protocols, books, articles, tips, reviews, web sites and advice
is welcome.
I know there are many steps where it can go wrong but I'd like to know
what experienced "in situ
hybridisers" judgeto be  the crucial  parameter.

Thank you for any help in advance.

Claire Fenech - PhD student - c.fenech at sghms(xxx) (remove x)
Dpt of Pharmacology
St George's Hospital Medical School
London - UK

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