Dnase treatment of RNA

Jim Wick wick at molbiores.com
Thu Sep 17 06:28:44 EST 1998


10x DNase Buffer: 500 mM Tris-HCl, pH 7.5, 10 mM MgCl2, 1 mg/ml BSA.
You can heat kill the DNase at 70C/10 min.and use the RNA immediately.

Jim Wick
Molecular Biology Resources, Inc.
Milwaukee, WI 53132

Narayanan Karthikeyan wrote in message
<36002FCB.90B81357 at ccvax.sinica.edu.tw>...
>Hi everyone
>Do anyone know the right buffer to go along with the Dnase in the
>treatment of RNA. And also, is it  neccessary to do  phenol:chloroform
>extraction after the treatment, in case I am using the Dnase treated RNA
>for making the double stranded c-DNA synthesis.
>Any help is appreciated
>Sincerely
>Narayanan Karthikeyan
>Dep. Biochem&Mol.Biol.,
>ETSU,
>TN, USA





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