Horrendous background problem on chemiluminescent westerns

Lou Cantolupo lou_cantolupo at GENVEC.COM
Thu Sep 17 13:51:06 EST 1998

As crazy as it sounds, I've been using the ECL+ kits and have
noticed--real science coming up here--that rabbit polyclonals give me a WAY higher
background than mouse.  I've yet to find out why.  Also of note, with these
rabbit polyclonals, the blots appear inverted.  Weird....


Lou Cantolupo
GenVec, Inc.
Department of Molecular Virology
12111 Parklawn Drive
Rockville, MD, USA  20852
lou_cantolupo at genvec.com

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