Horrendous background problem on chemiluminescent westerns

Bernard P. Murray, PhD bpmurray*STUFFER* at socrates.ucsf.edu
Fri Sep 18 15:31:07 EST 1998


In article <Pine.SGI.3.91.980918144907.11674A-100000 at tuna.biochem.mpg.de>,
Michael <blaesse at biochem.mpg.de> wrote:

> On 17 Sep 1998, Lou Cantolupo wrote:
> 
> > As crazy as it sounds, I've been using the ECL+ kits and have
[snip]

> 
> I just repeat an answer I wrote a week ago. 
> 
> Rabbit polyclonals seem to recognize unspecifically positiv charged 
> proteins. 
> I solved this problem by using acetylated BSA
[snip]

Aha!  This probably explains why 3% gelatin seems to be a better
blocking agent than 3% BSA on some occasions.  I hadn't tried to
link this to the species used to raise the primary or secondary
antibodies but maybe I should go back and look.
     Bernard
-- 
Bernard P. Murray, PhD
Dept. Cell. Mol. Pharmacol., UCSF, San Francisco, USA



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