separation of ssDNA from dsDNA

[Bernard P. Murray, PhD]

In article <6tunbt$3d2$1 at orthanc.reference.com>, j.migeon at cerep.com wrote:

> If anyone knows how to effectively separate single-stranded
> DNA from double stranded DNA (no gels),  I would be grateful
> for any suggestions.
> 

This will probably depend on the size(s) of the DNA.
A definite possibility is hydroxyapatite chromatography.
You may also be able to do it by density ultracentrifugation.
     I hope that this is a start,
          Bernard
-- 
Bernard P. Murray, PhD
Dept. Cell. Mol. Pharmacol., UCSF, San Francisco, USA