Tth RT-PCR

Dr. Duncan Clark duncan at nospam.demon.co.uk
Fri Sep 18 03:45:15 EST 1998


In article <obrien-1709981805300001 at zolot02.med.upenn.edu>, Peter
O'Brien <obrien at pharm.med.upenn.edu> writes
>I'm doing RT on total RNA from bone marrow, and will be using Tth to get
>throughRNA secondary structures at the 5' end of my (predicted) cDNA.  The
>insert for Tth says it has a 2-3Kb limit and they recommend a 1kb limit
>for product size.  
>
>Can I do the RT with Tth and PCR with another enzyme(s) to increase the
>end product size?  In other words, is the Tth "limit" due to the RT or the
>PCR step?
>
>Thanks,
>
>Peter

My educated guess is the RT. Tth will quite happily PCR 10kb of lambda
and in mixes 40kb.

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk



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