Dr. Duncan Clark duncan at
Fri Sep 18 03:45:15 EST 1998

In article <obrien-1709981805300001 at>, Peter
O'Brien <obrien at> writes
>I'm doing RT on total RNA from bone marrow, and will be using Tth to get
>throughRNA secondary structures at the 5' end of my (predicted) cDNA.  The
>insert for Tth says it has a 2-3Kb limit and they recommend a 1kb limit
>for product size.  
>Can I do the RT with Tth and PCR with another enzyme(s) to increase the
>end product size?  In other words, is the Tth "limit" due to the RT or the
>PCR step?

My educated guess is the RT. Tth will quite happily PCR 10kb of lambda
and in mixes 40kb.

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382

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