Dr. Duncan Clark
duncan at nospam.demon.co.uk
Fri Sep 18 03:45:15 EST 1998
In article <obrien-1709981805300001 at zolot02.med.upenn.edu>, Peter
O'Brien <obrien at pharm.med.upenn.edu> writes
>I'm doing RT on total RNA from bone marrow, and will be using Tth to get
>throughRNA secondary structures at the 5' end of my (predicted) cDNA. The
>insert for Tth says it has a 2-3Kb limit and they recommend a 1kb limit
>for product size.
>Can I do the RT with Tth and PCR with another enzyme(s) to increase the
>end product size? In other words, is the Tth "limit" due to the RT or the
My educated guess is the RT. Tth will quite happily PCR 10kb of lambda
and in mixes 40kb.
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....
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