Tetramethylbenzidine (TMB) staining

Louis Hom lhom at OCF.Berkeley.EDU
Mon Sep 21 20:11:37 EST 1998


We're using TMB to stain cells labeled with antibody-HRP.  The
signal/noise ratio is great, but we're having a problem mounting our
specimens.  They all seem to cause rapid fading:  glycerol, nail polish,
DPX (Aldrich), TBS's Tissue Freezing Medium, Permount, TissueTek's OCT.
Any ideas? Most of them seem to be poly-ols, maybe that's a problem.
Maybe a silicon-based mountant?  TIA.
-- 
______________________________________________________________________________
Lou Hom >K'93			     
lhom at ocf.berkeley.edu		
http://www.ocf.berkeley.edu/~lhom/ 	    



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