Whole COS Cell SDS PAGE

Jongrak Kittiworakarn g3937506 at STUDENT2.MAHIDOL.AC.TH
Mon Sep 21 20:24:50 EST 1998


> Hi  Marcel:

I have never run the whole tissue culture cells, but I have the problems with
DNA in my sample.  Try this protocol. It worked well for me:

Chen M, Christen P. Removal of chromosomal DNA by Mg2+ in the lysis buffer: an
improved lysis protocol for preparing Escherichia coli whole-cell lysates for
sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Anal Biochem. 1997;
246(2): 263-264.

Hope this help

Jongrak

> Whole COS Cell SDS PAGE
>
> Marcel Tanudji (mtanudji at cyllene.uwa.edu.au)
> 20 Sep 1998 08:30:10 GMT
>
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> To: methods at net.bio.net
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> From: mtanudji at cyllene.uwa.edu.au (Marcel Tanudji)
>
> Subject: Whole COS Cell SDS PAGE
>
> Date: 20 Sep 1998 08:30:10 GMT
>
> Hi all,
>
> i was wondering if poeple run whole tissue cultered cells on SDS-Page.
> If so is there a problem with the genomic DNa precipitaiting out of the
> solution (after boiling in leamlli buffer) as this will make the samples
> harder to load. Also does this have an effect on the electrophoresis of
> your protein?
>
> --
> -=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-
> = Marcel Tanudji =
> = Dept Biochemistry, University of Western Australia =
> = Perth, Australia [email:mtanudji at cyllene.uwa.edu.au] =
> = =
> -=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-
>   ------------------------------------------------------------------------
>
>    * Next message: Joseph C. Bagshaw: "Re: Q:CIP effect on DNA"
>    * Previous message: : "Re: self made TA vectors"
>    * Next in thread: Jan van der Lee: "Re: Whole COS Cell SDS PAGE"
>    * Reply: Jan van der Lee: "Re: Whole COS Cell SDS PAGE"
>    * Reply: Rich Dudley: "Re: Whole COS Cell SDS PAGE"
>    * Reply: stebby at welchlink.welch.jhu.edu: "Re: Whole COS Cell SDS PAGE"




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