cloning a gene that has secondary structure

John Ladasky jladasky at pmgm.Stanford.EDU
Mon Sep 21 18:40:41 EST 1998


In article <3606B406.AD966CCD at pitt.edu>,
Rich Dudley  <rdudley+ at pitt.edu> wrote:
[snip]
>Why do you want to clone a 34 kDa gene into an expression vector?  I
>don't think pET vectors can have such large inserts.  You'll probably
>need a YAC, and yeast expression systems.  Also, what are you trying to
[snip]

	Huh?

	34 kDa is only about 350 amino acids or so, which translates into
a cDNA of about 1 kilobase.  This is well within the size limits of plas-
mid expression systems.


-- 
Rainforest laid low.
"Wake up and smell the ozone,"
Says man with chainsaw.					- John Ladasky



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