cloning a gene that has secondary structure
jladasky at pmgm.Stanford.EDU
Mon Sep 21 18:40:41 EST 1998
In article <3606B406.AD966CCD at pitt.edu>,
Rich Dudley <rdudley+ at pitt.edu> wrote:
>Why do you want to clone a 34 kDa gene into an expression vector? I
>don't think pET vectors can have such large inserts. You'll probably
>need a YAC, and yeast expression systems. Also, what are you trying to
34 kDa is only about 350 amino acids or so, which translates into
a cDNA of about 1 kilobase. This is well within the size limits of plas-
mid expression systems.
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Says man with chainsaw. - John Ladasky
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