Megapore dialysis protocol?

Max Søgaard tmms at mbio.aau.dk
Mon Sep 21 11:16:13 EST 1998


Dear netters.
I am looking for a protocol for purification of highly intact genomic
DNA from cultured lyphocytes. The DNA is to be used for long PCR
(20-30kb).
I found a ref. to a protocol called megapore dialysis - supposedly this
should give DNA of a superb quality..
The ref was:
Monforte et  al.
"Megabase genomic DNA isolation procedure for use in transgenic
mutagenesis assays"
Environ. Mol. Mutagen.  23:46 (1994)
I have no access to this journal and as far as I can tell it is not
on-line.
If you have it please mail me a copy.
email:
tmms at mbio.aau.dk
If you have any experiences with the megapore dialysis -  sharing them
with me would be appreciated greatly.

Hopefully yours
Max Soegaard




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