Building T7 promoters onto primers

Dr. Duncan Clark duncan at nospam.demon.co.uk
Mon Sep 21 06:51:34 EST 1998


Hi Folks,

I need to run a transcript off a 1kb PCR product purely as a means to
generate RNA as a test system for an RT reaction.

I know I can just stick a T7 promoter sequence on the 5' end of one of
my primers but is there any additional extra sequence after the promoter
that would aid/increase the amount of RNA produced? Just wondering. I'll
probably use an Ambion or Epicentre transcription kit.

Many thanks

Duncan 
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk



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