lsc at novo.dk
Tue Sep 22 09:47:17 EST 1998
> A simple(?) protein purification question:
> As a first step, I've been trying to concentrate via ultrafiltration my
> protein of interest from conditioned mammalian cell culture media. The
> BSA present in the medium appears to be aggregating, it's being retained
> even with a 500 kD cutoff membrane! It also appears to be taking my
> stuff with it. Aside from using serum-free/protein-free medium (and
> taking the BSA out of the equation), is there a known method for
> avoiding BSA aggregate formation (salt, pH, reducing agents) without
> mucking up most of the other proteins in the gemish?
If your protein is glycosylated, a lectin column (WGA or ConA) might be
good idea as a first step to get rid of BSA and lots of other things.
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