JS5 - any clues?
dspinella at chugaibio.com
Wed Sep 23 13:24:06 EST 1998
> Gudday peeps,
> Could anyone please point me at some information regarding E. coli strain
> JS5? We've looked at ATCC and various catalogues but cannot find anything
> about it.
Hi Richard. JS5 is a derrivative of JS4 whose construction is described
by Leonardo and Sedivy (Bio/Technol. 8: 841; 1990). The strain is sold
commercially as competent cells by Bio Rad Labs here in the States. It
is widely used for phage display work (the strain is F'+ and is among
the most competent for transformation with M13). The strain is also the
only F+ strain that does not have the SupE44 amber supressor and is
therefore non-permissive for the growth of constucts with amber stop
codons. This allows one to use it to generate M13 phage display
libraries with a biologic selection against non-recombinants. My lab
has published on this application (for details, see McConnell et al.,
Mol. Diversity 1: 165-176; 1995).
Anyway, I have the full genotype information for JS5 and will FAX it to
you if you want -- just e-mail me directly with a FAX number. You can
probably also get the genotype info from Bio Rad, maybe on their
website. Hope that helps. Cheers. --Dom Spinella
More information about the Methods