Building T7 promoters onto primers

Dr. Peter Gegenheimer PGegen at UKans.nolospamare.edu
Fri Sep 25 18:31:18 EST 1998


On Wed, 23 Sep 1998 15:41:25, ELADER at AMBION.COM wrote:

> 5'-TAATACGACTCACTATAGGG(AGGAGG)  Add this to the 5' end of your 5' primer to
> make a template for sense strand RNA. You can use the PCR product in the
> transcription reaction without further purification.The first transcribed
> base is the middle G in the triplet before the parenthesis. The bases in the
<snip>

 The first nucleotide of the RNA transcript corresponds to the _first_ G in the
above-mentioned triplet, i.e. the G immediately following ...ACTATA.

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