I need help: Subcloning
Rich Dudley
rdudley+ at pitt.edu
Fri Sep 25 07:16:37 EST 1998
You can buy de-methylases, but for all the trouble they are, you're just
better off with a dam-dcm- strain and start the preps again. You never
think to check for methylation before this happens, but now you'll never
forget. I know, I've been there.
Good luck!
rich
--
--- --- --- -- -- -- --- --- ---
Richard J. Dudley (rdudley+ at pitt.edu)
Research Specialist V
Dept. of Cell Biology and Physiology
University of Pittsburgh
http://www.cbp.pitt.edu
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