I need help: Subcloning

Rich Dudley rdudley+ at pitt.edu
Fri Sep 25 07:16:37 EST 1998


You can buy de-methylases, but for all the trouble they are, you're just
better off with a dam-dcm- strain and start the preps again.  You never
think to check for methylation before this happens, but now you'll never
forget.  I know, I've been there.

Good luck!

rich

--
--- --- --- -- -- -- --- --- ---
Richard J. Dudley (rdudley+ at pitt.edu)
Research Specialist V
Dept. of Cell Biology and Physiology
University of Pittsburgh
http://www.cbp.pitt.edu
---> search BIONET archives at http://www.bio.net <---

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