Microplate cell growth problem

Morten Lindow moli5279 at student.uu.se
Sun Sep 27 09:05:21 EST 1998


Just a thought:

What Br-isotope are you using? Outer cells will recieve less crossfire
radiation from the surrounding wells and therefore might be less growth
inhibited than the center cells.

I don't know what doses you use, but I have the experience with hamster
fibroblasts that even 1000 Bq/mL (C-14 thymidine) makes a detectable
decrease in growth rate.

Morten Lindow





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