tritonX100

Markus Schneemann schneema at cmgm.Stanford.EDU
Mon Sep 28 13:15:06 EST 1998


Hi all:

we have problems with our PCR - sensitivity - only the positive controls
with lots of DNA give a signal.
We suspect the contamination of the buffer for sample preparation with
DNAse - esp. Triton X-100.
Can peroxide from old triton-X100 bottles inactivate Taq-activity or
otherwise interfere with the PCR at a conc. of 1% in TritonX100?

Cheers,

Markus




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