DNA quantitation in agarose/acrylamide gels

Yang Sheng shengyang at USA.NET
Mon Sep 28 09:27:31 EST 1998


At 98-9-28 6:14:00, you wrote:
>
>Dear all,
>This question was probably asked about 1000 times, however:
>what is the most precise/fast/cheap technique to quantify intensity of
>DNA bands in agarose/non-den. acrylamide gels (SYBR green, Ethidium
>bromide etc.) or it is necessary to blot and hybridize the gel using
>radioactive probe and Phosphoimager?

Hi, all:

	I think the fastest and cheapest way is to dyed with EB and compare with the DNA molucule 
size marker. I have no idea about what is the precised way :-(

Young




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