Microplate cell growth problem
wgschech at med.uni-tuebingen.de
Mon Sep 28 07:17:00 EST 1998
I'ts probably the naturally occuring isotope mixture of Br. BrdU is
*not* radioactive; that's the reason why we are using it. The assay
is colorimetric: BrdU is incorporated into DNA instead of
Thymidine. BrdU then is recocgnized by an antibody, this antibody is
labelled with peroxidase, peroxidase cleaves superoxide wich then
turns a chemical into a blue dye.
But thanks for the hint on 14C doses.
> Just a thought:
> What Br-isotope are you using? Outer cells will recieve less
> crossfire radiation from the surrounding wells and therefore might
> be less growth inhibited than the center cells.
> I don't know what doses you use, but I have the experience with
> hamster fibroblasts that even 1000 Bq/mL (C-14 thymidine) makes a
> detectable decrease in growth rate.
> Morten Lindow
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