help: c oning PFU pcr fragment

Mr. G. Morley gmorley at hgmp.mrc.ac.uk
Tue Sep 29 08:09:09 EST 1998


Hi all,
		I am having trouble cloning a some PFU pcr generated
products. This enzyme should produce a blunt ended fragment which
I have kinased. However it repeatedly fails to clone into a blunt
ended vector that has been CIP treated. Any suggestions? the fragments
are purified after kinase treatment by removal from LMP gel. Do the
fragments require phenoling?
Any suggestions would be appreciated as it is driving me nuts.
Thanks in advance.
Gary morley
g.morley at ucl.ac.uk




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