protein production and purification

Cornelius Krasel krasel at wpxx02.toxi.uni-wuerzburg.de
Wed Sep 30 12:19:28 EST 1998


B.Faucon <B.Faucon at starnet.fr> wrote:
> What is the best kit for the production of protein??? and in what
> system, E.coli, insect cells or eucaryotic system? ( my protein is a
> receptor ....)

It depends on your protein. If your protein is a GPCR, forget about
bacteria. If you want to purify it, insect cells are probably easier
to cultivate in large amounts than higher eukaryotic cells (like 293
cells).

> In the same way, does the his tag work well?

Usually yes.

--Cornelius.

-- 
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: phak004 at rzbox.uni-wuerzburg.de  SP4 */
/* "Science is the game we play with God to find out what His rules are."  */



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