I need help: Subcloning

Chris Boyd chrisb at hgu.mrc.ac.uk
Wed Sep 30 05:16:48 EST 1998


Rich Dudley (rdudley+ at pitt.edu) wrote:
: You can buy de-methylases, but for all the trouble they are, you're just
: better off with a dam-dcm- strain and start the preps again.  You never
: think to check for methylation before this happens, but now you'll never
: forget.  I know, I've been there.

I'm sceptical -- where can you buy demethylases? (Also, please can you
reconfigure your posting set-up so your messages aren't filled up with
MIME detritus?)

Best wishes,
--
Chris Boyd                      | from, but not \ MRC Human Genetics Unit,
Christopher.Boyd at hgu.mrc.ac.uk  | on behalf of  /  Western General Hospital,
http://www.hgu.mrc.ac.uk/Users/Christopher.Boyd \   Edinburgh, EH4 2XU, SCOTLAND



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