Electroporator for yeast

Tobias Straub tobias.straub at mail.uni-wuerzburg.de
Tue Apr 6 13:33:04 EST 1999


The e.coli pulser works fine for S. cerevisiae and pombe.

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In article <1537895B1173D1118D9300A0246212B6B9F580 at MCDC-ATL-4>, jby6 at cdc.gov
("Yokosawa, Jonny") wrote:


> Hi everybody,
> We are interested in buying an electroporator, probably the E. coli Pulser
> from Bio-Rad, but we want to use it for yeast transformation. However, the
> technical support said that it is not really recommended for that, although
> in the catalog it says that we can get 10^7 transformants/ug. Does anybody
> know if this equipment is good enough for yeast transformation? Or should we
> consider getting a better (and more expensive) electroporator?
> Thank you.
> Jonny Yokosawa
> jby6 at cdc.gov
> Centers for Disease Control and Prevention
> DVRD - Hepatitis Branch MS A-33
> 1600 Clifton Rd. NE
> Atlanta, GA    30333
> USA
> 



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