E2/ER responsive reporter plasmids

smf smfestin at mindspring.com
Tue Apr 13 19:29:11 EST 1999



Can anyone offer some advice regarding construction of ER stimulated
transcription from a (luciferase) reporter plasmid?  My goal is to test
non-competitive, anti-estrogenic molecules for interference with this
pathway.  Any help would be much appreciated, especially concerning
e2-responsive promoter constructs, vectors and transfection methods.

Thanks,

-Steve








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