How do they find new RE?
Dr. Duncan Clark
Duncan at nospam.demon.co.uk
Wed Apr 14 09:37:10 EST 1999
In article <Pine.SGI.3.94.990414163521.17694D-100000 at leonardo.ls.huji.ac
.il>, Yoram Gerchman <yoramg2 at LEONARDO.LS.HUJI.AC.IL> writes
>Just wondering, how dose companies find new Restriction and DNA modifying
For RE's in theory it is very simple, in practice 99.9% found are
isoschizomers. Basically assay aliquot of lysed test strain supernatant
with umpteen bacteriophage DNA's in a restriction digest with say Medium
slat RE buffer at appropraite temp. for appropriate time. If one or more
cut then purify and identify. The last two hundred or so isolates we
have found with RE's have not had new specificities just isoschizomers.
Some potentially useful ones but still iso's.
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....
More information about the Methods