plasmid from HB101 to DH5alpha

Bernard P. Murray, PhD bpmurray*STUFFER* at socrates.ucsf.edu
Thu Apr 22 18:20:38 EST 1999


In article <j91w+FAketH3EwKn at genesys.demon.co.uk>, "Dr. Duncan Clark"
<Duncan at genesys.demon.co.uk> wrote:

> In article <nawotka-ya023180002104992336170001 at news.earthlink.net>,
> Kevin Nawotka <nawotka at earthlink.net> writes
> >Is there a problem transferring plasmids between these two
> >strains 
 
> Unfortunately for you - no. Looks like deletions or contamination or
> your plasmid in the minipreps is not ccc and therefore runs differently
> on a gel.
> Duncan

Yes, this may be the reason the plasmid was in HB101 in
the first place as this strain has a reputation for
maintaining some problem plasmids more stably than common
strains (DH5alpha, JM109 etc.).  If your construct has
repetitive sequences within it this might explain the
selective stability in HB101.
     Bernard
-- 
Bernard P. Murray, PhD
Dept. Cell. Mol. Pharmacol., UCSF, San Francisco, USA



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