reliable plasmid cloning of pcr products

Daniel Schlieper aeg17 at dialog.rrz.uni-koeln.de
Fri Apr 23 03:12:47 EST 1999


Hello Bruce,

Roche's "PCR cloning kit (blunt end)" is reliable in my hands. It
works for Taq generated PCR products as well. When they are long 
(> 5 kb) they need to be polished. 

Best regards, Daniel

fishgen at vt.edu ("Bruce  J. Turner") writes:

> 
> Hello all.  I'm new to this list, so if an archive already exists about
> this topic, please simply refer me to it.
> 
> We have been using several different kits for the cloning of ordinary pcr
> products, generally less than 1 ib in length.  Each of these has worked
> once or twice, but it has been very hard to get consistent, reliable
> results over the long term. [...]

-- 
Daniel Schlieper                      Institut fuer Biochemie
                                      Zuelpicher Strasse 47
Daniel.Schlieper at Uni-Koeln.De         Universitaet zu Koeln 
Tel.: +49 221 470-6443, Fax: -5092    D-50674 Koeln, FRG




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