GST purification

Cornelius Krasel krasel at
Fri Apr 30 04:51:20 EST 1999

In bionet.molbio.methds-reagnts Ian Mc <i.mcfarlane at> wrote:
> Which form of glutathione do I use to elute my protein from
> glutathione-agarose beads? The oxidised form or the reduced form???

The reduced form, which is usually also healthier for your protein
(if it is oxidation-sensitive, you can kill it with the oxidized form.
GST itself is also oxidation-sensitive).


/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: phak004 at  SP4 */
/* "Science is the game we play with God to find out what His rules are."  */

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