Is DNA in agarose gels damaged by 312 nm UV-light??

Bruno Cenni cenni at cellbio.unige.ch
Fri Apr 30 05:33:54 EST 1999


We use such a light box since about a year for cloning and it works perfect. It
has plain visible blue light and you simply need an orange filter to view EtBr
stained DNA

Bruno

Jörgen Ullberg wrote:

> The product mensioned by Wolfgang might have been the "The
> dark reader" by Clare Chemical Research which have a non
> UV-light table said to detect DNA in the 100 pg range.
> WWW.Clarechemical.com. So far as I know it is fully possible
> to use DNA from 312 nm UV-light exposere for
> sequenceing.
>
> Jorgen
>
> "Wolfgang Schechinger"
> <Wolfgang.Schechinger at med.uni-tuebingen.de>
> wrote:
>
> > I wouldn't suppose that damage is so severe that you can't use your DNA
> > for sequenceing anymore but ligation/tranmsformation efficiency is said to
> > decrease severely. Recently, I saw a flyer from a companay I don't
> > remember unfortunately: they sell a transilluminator that works with blue
> > light (not: UV). Since EtBr easily may be excited with 488nm it should
> > work well without the damages caused by the UV richer in energy. The
> > device seems to be a household fluorescent lamp equipped with akind of
> > blue glass (cobalt stained glass?) in order to transmit only blue light
> > but to hold back orange and so.
> >
>
> > Wolfgang
> >
> >
>
> -- Jorgen

--
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Bruno Cenni, Ph.D.
University of Geneva
Dept. of Cell Biology
Sciences III
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cenni at cellbio.unige.ch
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