DNA sterilization before transfection

Ned Mantei mantei at cell.biol.ethz.ch
Mon Aug 2 01:58:22 EST 1999


In article <3.0.1.32.19990802155118.006ee88c at pop3.unsw.edu.au>,
r.gay at unsw.edu.au ("Dr. Robert Gay") wrote:

> Hi
> 
> I'm looking for an alternative way of sterilizing DNA before transfection.

Add about 5 ul of chloroform to DNA. Let stand about 10 min or longer.
Transfer aqueous phase to a rather large tube (e.g., 15-ml) with loose
cap, and let stand about 30 min or more so the chloroform evaporates.

-- 
Ned Mantei
Dept. of Cell Biology, Swiss Federal Institute of Technology
CH-8093 Zurich, Switzerland



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