Frank O. Fackelmayer
Frank.Fackelmayer at uni-konstanz.de
Mon Aug 2 07:03:19 EST 1999
For dialysis of small volumes, we put a drop of the stuff (between 10 and
100microliters) onto the hydrophobic (shiny) side of a Millipore VS filter
(0.025micrometer pores), floating on 20 to 50m buffer in a plastic petri dish.
Let stand undisturbed for 2h in the cold, then take up the drop with a pipette
Hope this helps,
Dooyeon Kim wrote:
> Does anybody tell me about the "drop-dialysis" method?
> I guess that it is the method of dialyzing small volume of
> DNA or protein samples but I do not have the slightest idea
> about the procedures and appratus(if it is commercially available).
> Please answer me. Any information regarding this question will be
> greatly appreciated.
> Ajou medical school Dooyeon Kim ......................
> Pharmacology Lab. dykim at bioneer.kaist.ac.kr
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