MTT vs.3H Thymidine

Pianoman skim7 at po-box.mcgill.ca
Mon Aug 2 10:50:46 EST 1999


Hello Experts,

In our lab, there is a little debate on the method and I wish anyone
can give us idea.

We are testing the effect of some reagents on cell proliferation and
DNA synthesis.  We did some cell counting experiment using
hemocytometer.  We are interested in DNA synthesis and  if possible,
ribonucleotide reductase activity.

At the point we did the experiment using hemocytometer, what is better
method between MTT and 3H thymidine uptake?  Which one can we  draw
more conclusion out of data with?

I would really appreciate any kind of information.



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