cjfields at jove.acs.unt.edu
Mon Aug 2 11:03:12 EST 1999
"Chong Wai Yin( Zhang Weixian)" wrote:
> Hi! Can anyone tell me when I will need to use Pfu instead of Taq polymerase
> during PCR? Also what makes a good polymerase when running PCR especially
> when I'm working on developing a PCR-based Rapid diagnostic Kit?? Thank you
> very much.
> Kelvin Chong
I use Pfu only when I'm interested in cloning the fragment. Pfu has one
of the best proofreading capabilities, but it gives a slower elongation
rate (500 bp/min). If you are using PCR for diagnostic purposes only, I
would use something else with a much faster rate.
C. J. Fields
Graduate Student, Dept. of Biological Sciences
The University of North Texas
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