RNA contamination in Southern Blot

Richard Moore rmoore at doppel.ucsf.edu
Mon Aug 2 14:10:11 EST 1999


I usually put a small amount of RNase in with my enzymes when I cut
g.DNA. Try 1ul of a 10mg/ml stock in with a 50-100ul digest. The RNase
is also reputed to help stabilise the restriction enzyme in much the
same way as adding BSA does.

Richard C Moore, PhD
Prusiner Lab
University of California San Francisco
Tel: (415) 502 1949
Fax: (415) 476 8386

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