Drop-dialysis, help

Jeff Bingam jbiingam at hotmail.com
Mon Aug 2 18:26:06 EST 1999


Does that really work?   How can you determine that salts are removed
during this kind of dialyis?  The amount of the volume to be dialyzed
is too small to determine its condutivity after dialysis.   BTW, what
is the molecular cut-off ranges?   I am just curious.
jeff

On Mon, 02 Aug 1999 14:03:19 +0200, "Frank O. Fackelmayer"
<Frank.Fackelmayer at uni-konstanz.de> wrote:

>Hi,
>For dialysis of small volumes, we put a drop of the stuff (between 10 and
>100microliters) onto the hydrophobic (shiny) side of a Millipore VS filter
>(0.025micrometer pores), floating on 20 to 50m buffer in a plastic petri dish.
>Let stand undisturbed for 2h in the cold, then take up the drop with a pipette
>again.
>
>Hope this helps,
>Frank




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