Cloning multiple genes on a vector

Robyn Roth RROTH at CSIR.CO.ZA
Wed Aug 4 09:34:45 EST 1999

as anyone had any experience with cloning multiple (2-4) genes into a
Saccharomyces vector?  Are there problems with repeating promoter
and terminator sequences, and are there any sources for vectors
containing 2 or more promoter-terminator cassettes?

Any info/advice would be greatly appreciated!


Robyn Roth
Research Scientist, Biotechnology Programme
CSIR Division of Bio/Chemical Technologies
Private Bag X2
1645 Modderfontein
South Africa
tel:  +27-11-605 2264
fax:  +27-11-608 3020
e-mail:  rroth at


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