Cloning multiple genes on a vector

D. KIM dkim at NMSU.Edu
Wed Aug 4 10:08:45 EST 1999


You might want to do a literature search for "bicistronic expression".
There are a few commercially-available bicistronic expression vectors,
designed for mammalian cells.  Perhaps the same technique can be applied
to yeast.

Daniel Kim


Robyn Roth <RROTH at CSIR.CO.ZA> wrote:
: as anyone had any experience with cloning multiple (2-4) genes into a
: Saccharomyces vector?  Are there problems with repeating promoter
: and terminator sequences, and are there any sources for vectors
: containing 2 or more promoter-terminator cassettes?

: Any info/advice would be greatly appreciated!

: Thanks


: Robyn Roth
: Research Scientist, Biotechnology Programme
: CSIR Division of Bio/Chemical Technologies
: Private Bag X2
: 1645 Modderfontein
: South Africa
: tel:  +27-11-605 2264
: fax:  +27-11-608 3020
: e-mail:  rroth at csir.co.za

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