plasmid prep kits?
colin at pombe.usask.ca
Thu Aug 5 11:33:24 EST 1999
In article <7oc9b3$dg9$1 at nnrp1.deja.com>, rprestonic at my-deja.com wrote:
> If this is a FAQ, let me know, but I'm wondering if
> there's a good medium-scale (100-500ml) plasmid prep
> kit in use these days, or is CsCl still the way to go.
> I haven't cloned since the stone age ten years ago and
> I suspect there might be improvements (convenience,
> purity) since then? ;-)
> Rob Preston
> Pgh PA
I've used many different mid-size prep kits over the years and we've gone
back to PEG-NaCl preparation. We get good yields and it works for our
applications. If you need supercoiled DNA, say for
transfection/transcription assays, you might want to CsCl purify still.
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