T.D. Laing tdlaing at nospam.dres.dnd.ca
Mon Aug 9 09:14:22 EST 1999

In article <7ojnet$4pu$1 at inf6serv.rug.ac.be>, "B."
<Ben.Wielockx at rug.ac.be> wrote:

> Hi,
> Can someone tell me how a pro-form of a matrix metalloprotease (MMP) can be
> active during zymography. You can see the clear zone of the pro and
> activated form. Why is that?

For a zymogram, the sample is run in a non-denaturing SDS-polyacrylamide
gel with incorporated substrate.  When you wash out the SDS, the pro-form
of the MMP doesn't fold completely into its original conformation, so it
leaves the enzyme active site open and available to cleave substrate.

Hope this helps,


T.D. Laing
tdlaing at dres.dnd.ca

Remove "nospam" from my e-mail address in the header to reply.

My opinions are my own and do not reflect those of anyone else's.  They'd probably prefer it that way.

More information about the Methods mailing list