tdlaing at nospam.dres.dnd.ca
Mon Aug 9 09:14:22 EST 1999
In article <7ojnet$4pu$1 at inf6serv.rug.ac.be>, "B."
<Ben.Wielockx at rug.ac.be> wrote:
> Can someone tell me how a pro-form of a matrix metalloprotease (MMP) can be
> active during zymography. You can see the clear zone of the pro and
> activated form. Why is that?
For a zymogram, the sample is run in a non-denaturing SDS-polyacrylamide
gel with incorporated substrate. When you wash out the SDS, the pro-form
of the MMP doesn't fold completely into its original conformation, so it
leaves the enzyme active site open and available to cleave substrate.
Hope this helps,
tdlaing at dres.dnd.ca
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