Effect of SDS on hybridization?
nicholas_theodorakis at urmc.rochester.edu
Mon Aug 9 10:22:29 EST 1999
In article <7of83f$b2s$1 at bubba.NMSU.Edu>,
D. KIM <dkim at NMSU.Edu> wrote:
> What effect, if any, would 0.5% to 1% SDS have on DNA/RNA hybridization,
> if salt conditions and temperature are kept unchanged?
> I was thinking of adding SDS to the solutions for PolyA+ selections, to
> help protect the RNA from degradation. Would this really mess things up?
> Daniel Kim
> dkim at nmsu.edu
There should be only a minimal effect (from the salt from the SDS).
My old protocols for poly(A)+ selection of mRNA using oligo-dT cellulose
use SDS in the buffers, BTW.
| Nick Theodorakis |
| nicholas_theodorakis at urmc.rochester.edu |
| (previously theodorn at gusun.georgetown.edu) |
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