Use of membranes with DIG label

Steven Goldberg goldbers at bms.com
Mon Aug 9 12:09:00 EST 1999


I have been experiencing very high background using DIG-labeled
probes.  Hybridization and detection are performed using solutions
purchased from Boehringer-Mannheim (including EasyHyb) with the
recommended amount of probe.  The only item not from B-M are my
membranes.  I have been using Hybond N+.  Could this be part of the
problem?  



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