stability of plasmid

Terry Lau tcklau at yahoo.com
Mon Aug 9 21:49:25 EST 1999


Hi,

Recently, I clone a insert(about 2.5k) by T/A cloning and I would like
to cut the insert from the T/A vector. I inoculate the cell from the
glycerol stock and grow in LB-AMP broth with shaking overnight. After
miniprep and restriction digestion, I only obtain a small smear on the
gel. Therefore, , I should retransform the plasmid every time. I don.t
know what is the problem. Please give me any suggestion.

Thanks a lot!




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