stability of plasmid
Wolfgang.Schechinger at med.uni-tuebingen.de
Tue Aug 10 05:48:06 EST 1999
Seems like DNAse to me (in any buffer or enzyme) -> check it
this way: incubate aliquots of a plasmid you know that it's ok with
the solutions to test. don't forger to test electrophoresis buffers.
> From: Terry Lau <tcklau at yahoo.com>
> Subject: stability of plasmid
> Date: Tue, 10 Aug 1999 10:49:25 +0800
> Organization: The Hong Kong Polytechnic University
> To: methods at hgmp.mrc.ac.uk
> Recently, I clone a insert(about 2.5k) by T/A cloning and I would
> like to cut the insert from the T/A vector. I inoculate the cell
> from the glycerol stock and grow in LB-AMP broth with shaking
> overnight. After miniprep and restriction digestion, I only obtain a
> small smear on the gel. Therefore, , I should retransform the
> plasmid every time. I don.t know what is the problem. Please give me
> any suggestion.
> Thanks a lot!
usual disclaimers apply * This message is RNAse free - please don't touch!
Dr. Wolfgang Schechinger
University of Tuebingen, Germany
email: wgschech at med.uni-tuebingen.de * wwWait: http://www.medizin.uni-tuebingen.de/~wgschech/start.htm
*unsolicited mail is *NOT* appreciated
More information about the Methods