Question on Plasmid Cloning

Andrew Ippolito aji97 at acsu.buffalo.edu
Tue Aug 10 07:28:30 EST 1999


Student to student:
    I believe that your fragments are in such access to the plasmid,
that it doesn't really matter if self-ligating species form, because you will
also get a ton inserted into your vector - it's all probabilities and amounts -
You have to keep in mind the millions of molecules present, and the fact
that you really only need 1 to penetrate into a cell and be
properly replicated in order for a colony to form.
Hope that made sense (and was accurate :)
-Andrew




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